Photoreduction and Photophosphorylation with Tris-Washed Chloroplasts
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چکیده
منابع مشابه
Photoreduction and Photophosphorylation with Tris - Washed Chloroplastst
The artificial electron donor compounds p-phenylenediamine (PD). N, N, N', N'tetramethyl-p-phenylenediamine (TMPD), and 2,6-dichlorophenol-indophenol (DCPIP) restored the Hill reaction and photophosphorylation in chloroplasts that had been inhibited by washing with 0.8 M tris (hydroxymethyl)aminomethane (tris) buffer, pH 8.0. The tris-wash treatment inhibited the electron transport chain betwee...
متن کاملFluorescence and delayed light emission in Tris-washed chloroplasts.
Yamashita and Butler [I] have shown that extraction of chloroplasts with a high concentration of Tris (0.8 M, pH 8.0) eliminates flow of electrons from water to nicotinamide adenine dinucleotide phosphate (NADP+)* or other Hill oxidants. They suggest that washing with Tris causes a block on the water side of the electron transport chain. Addition of electron donors such as reduced phenylene dia...
متن کاملPhotoreduction of 2,6-dichlorophenolindophenol by diphenylcarbazide: a photosystem 2 reaction catalyzed by tris-washed chloroplasts and subchloroplast fragments.
The use of diphenylcarbazide as an electron donor coupled to the photoreduction of 2,6-dichlorophenolindophenol by tris-washed chloroplasts or subchloroplast fragments provides a simple and sensitive assay for photosystem 2 of chloroplasts. By varying the concentration of tris buffer at pH 8.0 during an incubation period it is shown that the destruction of oxygen evolution activity is accompani...
متن کاملPhotooxidation by Photosystem II of Tris . washed Chloroplasts ' Takashi
Irradiation of tris-washed chloroplasts with moderate intensities of red light caused a partial bleaching of chloroplast pigments and an inhibition of the hydroquinonesupported photoreduction of NADP. The presence of an electron donor for photosystem 2 (PS2) during the irradiation prevented the bleaching and inhibition. It is concluded that the strong oxidant produced by PS2 accumulates in tris...
متن کاملPhotooxidation by photosystem II of tris-washed chloroplasts.
Irradiation of tris-washed chloroplasts with moderate intensities of red light caused a partial bleaching of chloroplast pigments and an inhibition of the hydroquinone-supported photoreduction of NADP. The presence of an electron donor for photosystem 2 (PS2) during the irradiation prevented the bleaching and inhibition. It is concluded that the strong oxidant produced by PS2 accumulates in tri...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1968
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.43.12.1978